TGF-β LAP Degradation Products, a Novel Biomarker and Promising Therapeutic Target for Liver Fibrogenesis (2024)

While there are many blood and/or tissue biomarkers as well as algorithms clinically used to assess hepatic fibrosis, a good biomarker and therapeutic target of hepatic fibrogenesis, which reflects prefibrotic changes, has not been established. The most fibrogenic cytokine, transforming growth factor (TGF)-β, is produced as a latent complex, in which TGF-β is trapped by its propeptide. On the surface of activated hepatic stellate cells, plasma kallikrein activates TGF-β by cleaving latency-associated protein (LAP) between the R⁵⁸ and L⁵⁹ residues, releasing active TGF-β from the complex. We made specific antibodies that recognize neo-C-terminal (R⁵⁸) and N-terminal (L⁵⁹) ends of LAP degradation products (LAP-DPs) and found that LAP-DPs may serve as a novel surrogate marker of TGF-β activation—namely, generation of active TGF-β—and is thus a therapeutic marker for TGF-β-mediated liver fibrogenesis in patients and can also be used to monitor effects of anti-fibrogenic factors or compounds for discovery of a novel anti-fibrosis drug.

Keywords:

Biomarkers, Hepatic fibrogenesis, TGF-β, LAP, Latent TGF-β activation, Hepatic stellate cells, Plasma kallikrein, LAP-DP, Drug discovery, Anti-fibrosis drug

Abbreviations

ECM: Extracellular matrix
HSCs: Hepatic stellate cells
αSMA: α smooth muscle actin
TGF-β1: Transforming growth factor-β1
LAP: Latency associated protein
SLC: Small latent complex
LTBP: Latent TGF-β binding protein
LLC: Large latent complex
PLN: Plasmin
PLK: Plasma kallikrein
LAP-DP: LAP degradation products
LAP β1: TGF-β1 LAP
BDL: Bile duct ligation
HBV: Hepatitis B virus
HCV: Hepatitis C virus
NASH: Non-alcoholic steatohepatitis

Introduction of Liver Fibrogenesis

Hepatic fibrosis is the excessive accumulation of extracellular matrices (ECM; mainly collagen) in the perisinusoidal space (or space of Disse) in the liver, and an important pathological step developing from chronic hepatitis to liver cirrhosis irrespective of etiologies [1], whereas hepatic fibrogenesis means fibrosis progression or an ongoing reaction producing excessive ECM, sometimes nonsymptomatic, in the liver [2]. While there are many blood and/or tissue biomarkers as well as algorithms clinically used to assess hepatic fibrosis [3–7], the gold standard is still scoring of stained collagen fibers in the biopsy sample [3]. However, biopsy is invasive and risky. Imaging techniques including ultrasound elastography have been developed [3]. In contrast, a good biomarker and therapeutic target of hepatic fibrogenesis, which reflects prefibrotic changes, has not been established [2,3]. Therefore, development of a noninvasive biomarker for hepatic fibrogenesis, which will lead not only to establishment of a novel diagnosis useful to prevent liver fibrosis/cirrhosis, but also to acceleration of drug discovery and development against liver fibrosis, is in high demand [3].

Activation of Hepatic Stellate Cells

Hepatic stellate cells (HSCs) play a central role in the pathogenesis of hepatic fibrosis by virtue of their ability to undergo a process termed “activation” [1,2]. During this process, HSCs transform into myofibroblast-like cells accompanying several key phenotypic changes, which collectively increase extracellular matrix accumulation [1–3]. These include (1) cellular proliferation caused by upregulation of mitogenic cytokines and their receptors; (2) morphologic changes with loss of stored vitamin A droplets; (3) contractility caused by increased α smooth muscle actin (αSMA), which may constrict sinusoidal blood flow; and (4) fibrogenesis mainly caused by increased synthesis and release of collagen.

TGF-β and Its Activation Reaction

Among many cytokines and growth factors related to fibrogenesis, the most potent—and therefore the most “fibrogenic”—cytokine is the 25 kD hom*odimeric cytokine, transforming growth factor (TGF)-β [8]. The TGF-β family is composed of three subtypes (TGF-β1, TGF-β2, and TGF-β3), with biological properties that are nearly identical [8]. TGF-β is produced as an inactive latent complex, in which active TGF-β is trapped by its propeptide, latency-associated protein (LAP), and to exert its biological activities, it must be released from the complex [9]. This reaction is called activation of TGF-β (Fig. 1). TGF-β1 is produced as a 390-amino-acid precursor protein consisting of a signal peptide of 29 amino acids, an N-terminal LAP, and a C-terminal region that becomes the active TGF-β1 molecule, and each region is dimerized through S–S bonds. After processing by cleavage at R²⁷⁸–A²⁷⁹ by a furin-like protease, the LAP still non-covalently captures the active TGF-β1, forming small latent complex (SLC) and preventing active TGF-β1 from binding its cognate receptors [9]. The active TGF-β1 and the LAP hom*odimers are 25 kD and 75 kD, respectively. SLC is S–S bonded to another gene product, the latent TGF-β binding protein (LTBP), via C³³ residues, forming the large latent complex (LLC). This complex can be sequestered in the ECM (Fig. 1) [10] because LTBP is a member of an ECM protein family, fibrillin [11].

Fig. 1

TGF-β activation and signaling pathway. TGF-β is produced as a latent complex (LLC) composed of active TGF-β trapped by its propeptide LAP (SLC) and a matrix protein LTBP. Upon cleavage of LAP by proteases such as PLK, active TGF-β (more...)

Activation of latent TGF-β is performed through different mechanisms depending on the tissue and cell types and experimental conditions, and several molecules are known to activate TGF-β1 in animal models [12–21]. These include integrins [12–15], thrombospondin [16], and proteases, such as matrix metalloproteinases and serine proteases [17–21]. The integrin αvβ6 binds to and activates latent TGF-β and plays a role in regulating pulmonary inflammation and fibrosis as well as biliary fibrosis [12–15]. Thrombospondin 1 is another major activator of latent TGF-β, especially in the lung and pancreas, by binding to a defined site within LAP and inducing a conformational change in the latent complex [16]. In the normal liver, TGF-β is produced and secreted from sinusoidal endothelial cells and Kupffer cells (KCs, resident macrophages in the liver) at low levels. Elevated production of TGF-β was seen first in all cell types and then mainly in hepatocytes and HSCs after partial hepatectomy, whereas elevated production of TGF-β was seen solely in HSCs after inflammation and fibrosis [22]. TGF-β secreted from HPCs is entirely in the latent form, whereas TGF-β secreted from HSCs is 50–90 % in the active form [22]. Thus, HSCs are recognized as the major source of active TGF-β, namely the site of TGF-β activation, particularly in the damaged liver [22,23].

We have addressed a potential proteolytic mechanism for latent TGF-β activation in HSCs by surface plasmin (PLN) and plasma kallikrein (PLK) during the formation of hepatic fibrosis [20,21]. PLN releases latent TGF-β from the extracellular matrix and activates it by cleaving LAP from latent TGF-β molecules on the HSC surface [9,20]. Lyons et al. first reported that PLN digests LAP and activates TGF-β1 in vitro [24]. Using a protease inhibitor, camostat mesilate, we demonstrated that PLN and PLK are involved in the TGF-β1 activation associated with liver fibrosis and impaired liver regeneration in animal models [20,21]. However, it remained to be elucidated whether PLN- and/or PLK-dependent TGF-β1 activation also occurs during the pathogenesis of liver fibrosis in patients, as there was no good biomarker reflecting protease-dependent TGF-β1 activation reaction. To answer this question, we determined cleavage site within LAP and made specific antibodies that recognize LAP degradation products (LAP-DPs) bearing a neo-amino or carboxyl terminus [25].

TGF-β LAP-DP Serves as a Surrogate Marker for Its Activation Reaction

To identify the cleavage sites in LAP during latent TGF-β1 activation by PLN and PLK, recombinant human LAP β1 was digested with these proteases, the resultant fragments were separated by SDS-polyacrylamide gel electrophoresis (PAGE), and the N-terminal sequence of each LAP-DP was determined using a pulsed liquid protein sequencer Precise 494cLC, which revealed that PLN and PLK primarily cleave LAP β1 between the K⁵⁶ and L⁵⁷ residues, and the R⁵⁸ and L⁵⁹ residues, respectively, during proteolytic activation of latent TGF-β1 (Fig. 2) [25].

Fig. 2

Cleavage of K⁵⁶LRL⁵⁹ within LAP activates TGF-β. PLN and PLK cleave LAP between K⁵⁶–L⁵⁹ and R⁵⁸–L⁵⁹ residues, respectively, causing release of active TGF-β1 from the latent complex. The amino acid sequences around the PLN (more...)

To detect PLK-produced LAP-DPs, we made two monoclonal antibodies. One is the R58 antibody detecting N-terminal side LAP-DPs terminating on the R⁵⁸ residue (R⁵⁸ LAP-DPs), and another is the L59 antibody detecting C-terminal side LAP-DPs starting from the L⁵⁹ residue (L⁵⁹ LAP-DPs). We established techniques to detect each LAP-DP using these antibodies [25]. The R⁵⁸ LAP-DPs remaining in tissues or cell surfaces through S–S bonded LTBP can be detected mostly in αSMA-positive activated stellate cells in liver tissues from both fibrotic animals and patients by immunostaining with the R58 antibody, whereas the L⁵⁹ LAP-DPs were not detectable by immunostaining with the L59 antibody [25]. Figure 3 shows the results obtained from bile duct ligation (BDL) mice. These mice often exhibited granulomatous lesions (panel a), in which fibroblastic cells infiltrated and started ECM production (panel b). Importantly, the R⁵⁸ LAP-DPs were detected in granulomatous lesions prior to Sirius red positivity, namely before collagen accumulation (arrowheads in panel c). In contrast, L59 antibody failed to stain the L⁵⁹ LAP-DPs, although various antigen unmasking procedures were treated (panel d). We found that the L⁵⁹ LAP-DPs were released into the blood and could be measured by an ELISA using the L59 antibody (Hara et al., unpublished data). In panels e–h, nonparenchymal regions were recognized by antibody R58 (arrowheads in panel e), and mostly overlapped with αSMA-positive HSCs (arrowheads in panel f), but not with CD31-positive liver sinusoidal endothelial cells (panel g) nor with CD68-positive KCs (hepatic macrophages) (panel h). We further found that the R58 antibody detected TGF-β1/3 LAP-DPs but not TGF-β2 LAP-DPs because of the similarity and difference of the R58 side sequence, respectively (Fig. 2). Finally, we succeeded in detecting R⁵⁸ LAP-DPs in patients with chronic hepatitis B and C virus (HBV and HCV, respectively) infection categorized as A1F2 and A2F2, as well as in patients with non-viral hepatitis, such as autoimmune hepatitis and non-alcoholic steatohepatitis (NASH) [25]. A specific cell shape called a “crown-like structure” (CLS) has been referred to as a biomarker for NASH in both an animal model and patients [26]. Recently, we found that R⁵⁸ LAP-DPs positivity well matched the emergence of CLS [27].

Fig. 3

Emergence of TGF-β LAP-DPs in activated HSCs within pre-fibrotic areas in BDL models. Liver sections from BDL-operated mice were stained by HE (a) and Sirius red (b), and immunostained with R58 (c) and L59 (d) antibodies (scale bar = 50 (more...)

These data suggest the occurrence of a PLK-dependent TGF-β activation reaction in patients and indicate that the LAP-DP may be useful as a surrogate marker reflecting PLK-dependent TGF-β1/3 activation and subsequent fibrogenesis in the fibrotic liver both in animal models and in patients.

Conclusion and Future Subjects

The most fibrogenic cytokine, TGF-β, is produced as a latent complex, in which TGF-β is trapped by its propeptide, LAP. On the surface of activated HSCs, PLK activates TGF-β by cleaving LAP between the R⁵⁸ and L⁵⁹ residues, releasing active TGF-β from the complex. We made specific antibodies that recognize the neo-C-terminal (R⁵⁸) and N-terminal (L⁵⁹) ends of the LAP-DP, and found that the LAP-DP may serve as a novel surrogate marker of TGF-β activation—namely, generation of active TGF-β—and is thereby a therapeutic marker for TGF-β-mediated liver fibrogenesis in patients [25].

Utilizing LAP-DP antibodies, we are developing techniques to visualize the fibrogenic area by positron emission tomography (PET), planning to eliminate activated HSCs with pertussis toxin, and undertaking the challenge to solve the co-crystal structure of LAP and a LAP-DP targeting inhibitor, which binds to the LAP cleavage site, thereby inhibiting TGF-β activation and liver fibrosis in HBV-infected chimeric mice (Hara et al., unpublished data). The effectiveness of an inhibitor against the TGF-β activation reaction has been reported in the integrin-mediated activation of TGF-β [3, 15, 28]. LAP-DP is also used to monitor the effects of anti-fibrogenic factors or compounds for discovery of a novel anti-fibrosis drug. For example, we recently found that HCV NS3 protease mimics TGF-β2 and enhances liver fibrosis via binding to and activation of the TGF-β type I receptor, and that an anti-NS3 antibody raised against the predicted binding sites attenuates liver fibrosis in HCV-infected chimeric mice [29]. In this study, R58 LAP-DP staining nicely showed the anti-fibrogenic potentials of the anti-NS3 antibody.

The technique developed accelerates drug discovery targeting TGF-β-dependent fibrogenesis in patients suffering from chronic hepatitis.

Acknowledgments

This work was supported partly by the Program for Promotion of Fundamental Studies in Health Science of the National Institute of Biomedical Innovation (NIBL) and a grant from the Uehara Memorial Foundation, Japan (to S.K.), Research on the Innovative Development and the Practical Application of New Drugs for Hepatitis B (Principal investigator: Soichi Kojima; H24-B Drug Discovery-Hepatitis-General-003), provided by the Ministry of Health, Labor and Welfare of Japan.

References

1.: Bataller R, Brenner DA (2005) Liver fibrosis. J Clin Invest 115:209–218. [PMC free article: PMC546435] [PubMed: 15690074] [CrossRef]
2.: Friedman SL (2008) Mechanism of hepatic fibrogenesis. Gastroenterology 134:1655–1669. [PMC free article: PMC2888539] [PubMed: 18471545] [CrossRef]
3.: Schuppan D, Kim YO (2013) Evolving therapies for liver fibrosis. J Clin Invest 123:1887–1901. [PMC free article: PMC3635731] [PubMed: 23635787] [CrossRef]
4.: Kuno A, Ikehara Y, Tanaka Y, Ito K, Matsuda A, Sekiya S, Hige S, Sakamoto M, Kage M, Mizokami M, Narimatsu H (2013) A serum “sweet-doughnut” protein facilitates fibrosis evaluation and therapy assessment in patients with viral hepatitis. Sci Rep 3:1065. [PMC free article: PMC3545220] [PubMed: 23323209] [CrossRef]
5.: Ikeda K, Izumi N, Tanaka E, Yotsuyanagi H, Takahashi Y, f*ckushima J, Kondo F, f*ckusato T, Koike K, Hayashi N, Kumada H (2013) Fibrosis score consisting of four serum markers successfully predicts pathological fibrotic stages of chronic hepatitis B. Hepatol Res 43:596–604. [PubMed: 23131000] [CrossRef]
6.: Fallatah HI (2014) Noninvasive biomarkers of liver fibrosis: an overview. Adv Hepatol. Article ID 357287.
7.: Kazankov K, Barrera F, Møller HJ, Bibby BM, Vilstrup H, George J, Grønbaek H (2014) Soluble CD163, a macrophage activation marker, is independently associated with fibrosis in patients with chronic viral hepatitis B and C. Hepatology 60:521–530. [PubMed: 24623375] [CrossRef]
8.: Dooley S, ten Dijke P (2012) TGF-β in progression of liver disease. Cell Tissue Res 347:245–256. [PMC free article: PMC3250614] [PubMed: 22006249] [CrossRef]
9.: Dabovic B, Rifkin DB (2008) TGF-β bioavailability: latency, targeting, and activation. In: Derynck R, Miyazono K (eds) The TGF-β family. Cold Spring Harbor Laboratory Press, New York, pp 179–202.
10.: Breitkof K, Lahme B, Tag CG, Gressner AM (2001) Expression and matrix deposition of latent TGF-β binding proteins in normal and fibrotic rat liver and transdifferentiating hepatic stellate cells in culture. Hepatology 33:387–396. [PubMed: 11172340] [CrossRef]
11.: Zilberberg L, Todorovic V, Dabovic B, Horiguchi M, Couroussé T, Sakai LY, Rifkin DB (2012) Specificity of latent TGF-β binding protein (LTBP) incorporation into matrix: role of fibrillins and fibronectin. J Cell Physiol 227:3828–3836. [PMC free article: PMC3404192] [PubMed: 22495824] [CrossRef]
12.: Margadant C, Sonnenberg A (2010) Integrin-TGF-β crosstalk in fibrosis, cancer and wound healing. EMBO Rep 11:97–105. [PMC free article: PMC2828749] [PubMed: 20075988] [CrossRef]
13.: Shi M, Zhu J, Wang R, Chen X, Mi L, Walz T, Springer TA (2011) Latent TGF-β structure and activation. Nature 474:343–349. [PMC free article: PMC4717672] [PubMed: 21677751] [CrossRef]
14.: Henderson NC, Sheppard D (2012) Integrin-mediated regulation of TGFβ in fibrosis. Biochim Biophys Acta 1832:891–896. [PMC free article: PMC3573259] [PubMed: 23046811] [CrossRef]
15.: Patsenker E, Popov Y, Stickel F, Jonczyk A, Goodman SL, Schuppan D (2008) Inhibition of integrin αvβ6 on cholangiocytes blocks transforming growth factor-β activation and retards biliary fibrosis progression. Gastroenterology 135:660–670. [PMC free article: PMC3505071] [PubMed: 18538673] [CrossRef]
16.: Ribeiro SM, Poczatek M, Schultz-Cherry S, Villain M, Murphy-Ullrich JE (1999) The activation sequence of thrombospondin-1 interacts with the latency-associated peptide to regulate activation of latent transforming growth factor-β. J Biol Chem 274:13586–13593. [PubMed: 10224129] [CrossRef]
17.: Jenkins G (2008) The role of proteases in transforming growth factor-β activation. Int J Biochem Cell Biol 40:1068–1078. [PubMed: 18243766] [CrossRef]
18.: Du X, Shimizu A, Masuda Y, Kuwahara N, Arai T, Kataoka M, Uchiyama M, Kaneko T, Akimoto T, Iino Y, f*ckuda Y (2012) Involvement of matrix metalloprotease-2 in the development of renal interstitial fibrosis in mouse obstruction nephropathy. Lab Invest 92:1149–1160. [PubMed: 22614125] [CrossRef]
19.: Lyons RM, Gentry LE, Purchio AF, Moses HL (1990) Mechanism of activation of latent recombinant transforming growth factor beta 1 by plasmin. J Cell Biol 110:1361–1367. [PMC free article: PMC2116088] [PubMed: 2139036] [CrossRef]
20.: Okuno M, Akita K, Moriwaki H, Kawada N, Ikeda K, Kaneda K, Suzuki Y, Kojima S (2001) Prevention of rat fibrosis by protease inhibitor, Camostat Mesilate, via reduced generation of active TGF-β. Gastroenterology 120:1784–1800. [PubMed: 11375959] [CrossRef]
21.: Akita K, Okuno M, Enya M, Imai S, Moriwaki H, Kawada N, Suzuki Y, Kojima S (2002) Impaired liver regeneration in mice by lipopolysaccharide via TNF-α/kallikrein-mediated activation of latent TGF-β. Gastroenterology 123:352–364. [PubMed: 12105863] [CrossRef]
22.: Bissell DM, Wang SS, Jarnagin WR, Roll FJ (1995) Cell-specific expression of transforming growth factor-β in rat liver. Evidence for autocrine regulation of hepatocyte proliferation. J Clin Invest 96:447–455. [PMC free article: PMC185218] [PubMed: 7615817] [CrossRef]
23.: Friedman SL (1993) The cellular basis of hepatic fibrosis. N Engl J Med 328:1828–1835. [PubMed: 8502273] [CrossRef]
24.: Lyons RM, Keski-Oja J, Moses HL (1988) Proteolytic activation of latent transforming growth factor-β from fibroblast-conditioned medium. J Cell Biol 106:1659–1665. [PMC free article: PMC2115066] [PubMed: 2967299] [CrossRef]
25.: Hara M, Kirita A, Kondo W, Matsuura T, Nagatsuma K, Dohmae N, Ogawa S, Imajoh-Ohmi S, Friedman SL, Rifkin DB, Kojima S (2014) LAP degradation product reflects plasma kallikrein-dependent TGF-β activation in patients with hepatic fibrosis. Springerplus 3:221. [PMC free article: PMC4033717] [PubMed: 24877031] [CrossRef]
26.: Itoh M, Kato H, Suganami T, Konuma K, Marumoto Y, Terai S, Sakugawa H, Kanai S, Hamaguchi M, f*ckaishi T, Aoe S, Akiyoshi K, Komohara Y, Takeya M, Sakaida I, Ogawa Y (2013) Hepatic crown-like structure: a unique histological feature in non-alcoholic steatohepatitis in mice and humans. PLoS One 8, e82163. [PMC free article: PMC3859576] [PubMed: 24349208] [CrossRef]
27.: Konuma K, Itoh M, Suganami T, Kanai S, Nakagawa N, Sakai T, Kawano H, Hara M, Kojima S, Izumi Y, Ogawa Y (2015) Eicosapentaenoic acid ameliorates non-alcoholic steatohepatitis in a novel mouse model using Melanocortin-4 receptor-deficient mice. PlosOne 10, e0121528. [PMC free article: PMC4376873] [PubMed: 25816330]
28.: Allison M (2012) Stromedix acquisition signals growing interest in fibrosis. Nat Biotechnol 30:375–376. [PubMed: 22565944]
29.: Sakata K, Hara M, Terada T, Watanabe N, Takaya D, Yaguchi S, Matsumoto T, Matsuura T, Shirouzu M, Yokoyama S, Yamaguchi T, Miyazawa K, Aizaki H, Suzuki T, Wakita T, Imoto M, Kojima S (2013) HCV NS3 protease enhances liver fibrosis via binding to and activating TGF-β type I receptor. Sci Rep 3:3243. [PMC free article: PMC3837337] [PubMed: 24263861] [CrossRef]